Journal: Scientific Reports

Article Title: An organ-on-chip device with integrated charge sensors and recording microelectrodes

doi: 10.1038/s41598-023-34786-5

Figure Lengend Snippet: Biocompatibility and electrophysiological proof of concept experiments: ( a , b ) HiPSC-derived cortical neurons differentiated (7 days) and matured (14 days) on-chip. After staining, \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\beta 3$$\end{document} β 3 tubulin-positive neurons (green) and synaptic vesicles (red) are visible. ( c ) Compatibility with Multichannel Systems MEA recording setup of the PCB and the chip. ( d ) Inset showing the chip and the well with medium and cells. ( e ) Single-electrode trace signal from the MEA recordings. ( f ) The addition of Picrotoxin (50 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upmu \hbox {M}$$\end{document} μ M ) caused an increase in fire rate.

Article Snippet: Lastly, the hiPSC-derived cortical neurons were matured and maintained in BrainPhys hiPSC Neuron kit media (05795, StemCell Technologies) for the remainder of the experiment.

Techniques: Derivative Assay, Staining

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: List of ELISA tests, cell proliferation, and protein extraction kits used. For ELISA tests, dilution of the supernatants or cell lysate samples used and detection limits are also reported.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Enzyme-linked Immunosorbent Assay, Protein Extraction

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: C-Kit (CD117) mRNA (a, b, c) and protein (d, e, f) expression after ESW treatment in primary bronchial fibroblasts of COPD patients (a, d), primary bronchial fibroblasts of control smokers (b, e), and bronchial epithelial cells (c, f). In bronchial epithelium (16HBE) c-Kit increased at mRNA (c) and protein (f) levels. In primary bronchial fibroblasts of COPD patients, c-Kit increased at protein level (d). T -test was used for comparative purposes, and p values are reported in the graphs.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing thyroid transcription factor-1 (TTF-1) expression (panels a, b), c-Kit (CD117) (c, d), and proliferating cell nuclear antigen (PCNA) (e, f) in the peripheral lung tissue of a representative patient with chronic obstructive pulmonary disease (COPD). Arrows indicate positively stained cells mainly located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Expressing, Staining

Journal: Canadian Respiratory Journal

Article Title: Extracorporeal Shock Waves Increase Markers of Cellular Proliferation in Bronchial Epithelium and in Primary Bronchial Fibroblasts of COPD Patients

doi: 10.1155/2020/1524716

Figure Lengend Snippet: Photomicrographs showing alveolar type II epithelial cells (TTF-1+ cells, red color) coexpressing c-Kit (CD117) (brown color) (a, b) and PCNA (brown color) (c, d) in the peripheral lung tissue of a representative patient with COPD. Positive double-stained cells can be recognized in the alveolar septa, even though their presence was only rarely observed. Arrows indicate positively stained cells located in the alveolar septa. Bars = 50 microns.

Article Snippet: c-Kit or SCFR (CD117) , Cloud-Clone Corp. , SEA121 Hu , 1 : 5 (PBS) , 0.61 ng/mL (1.56–100 ng/mL).

Techniques: Staining